Belmont SURFS

Purification of GST-Nup42 and Stimulation of Dbp5 ATPase Activity

We are studying the importance of specific proteins that aid the process of mRNA export. There are many proteins that assist mRNA export such as Nup42. I want to know how important is the Nup42 interaction with mRNA. By purifying Nup42, it will allow future studies to focus on mutations and purifications of proteins involved with mRNA exporting. We will be approaching our experiment by inserting specific plasmids into E. coli cells and additional tags. We will then look at activity using ATPase assays. We conclude that expression will be maximized if the proteins are purified. By seeing the importance of the protein activities, we can then begin to create a foundation for future biochemical research at Belmont.